1Step Magic SYBR Kit is a one-step solution for the quantitative analysis of RNA. The 2X qPCR mastermix contains all components necessary for a qPCR experiment: Hotstart DNA polymerase, dNTPs (with a blend of dTTP and dUTP to allow for the digestion with UNG), SYBR® Green and a passive reference dye, all supplied in a reaction buffer optimized for selective amplification of target sequences. For the conversion of RNA to cDNA before the amplification, VitaScript™ Reverse Transcriptase is provided as a 50X solution.
The 1Step formulation is a convenient solution as handling steps and hands-on time are reduced. Thereby, reproducibility and throughput are increased.
- Quantitative PCR
- Realtime PCR
- Genexpression Analysis
- High-throughput applications
|Polymerase||Taq DNA Polymerase|
|Reaction Speed||Fast / Standard|
|Product Size||200 / 600 reactions à 20 µl|
|Normalization||universal ROX™ concentration|
|Reaction Format||2X Mastermix, 50X VitaScript™ separately|
|Shipping Condition||Approved for shipment on wet or dry ice|
|Storage||-15°C to -25°C|
SKU 200 rxn / 20 µl: PCCSKU1201
SKU 600 rxn / 20 µl: PCCSKU1202