Our recently developed Indominus®-Polymerase is a next-generation designer proofreading polymerase that combines exceptional speed and efficiency with a high amplification fidelity. It is
an allround talent for many applications but especially recommended for high fidelity applications like cloning or NGS. In contrast to well-known market-leading products, Indominus® is no Sso7D-fusion protein but a whole new generation of proofreading polymerase.
Fig. : ~200 bp sequences of eleven target genes were amplified in recommended protocols and setups according to manufacturer’s guidelines. Reaction volume, number of cycles and amount of template were identical for all PCR kits. The template used here was cDNA synthesized from isolated HEK 293 RNA with the VitaScript® FirstStrand cDNA Synthesis Kit. Primers have been designed with the Primer3 Software.