VitaScript® 1Step RT-PCR Kit is a combined reverse transcription / PCR amplification system for the analysis of RNA with endpoint PCR. The optimized 1Step formula allows for reverse transcription and amplification of the target sequence to be performed in one reaction saving time and resources while being highly sensitive and efficient. Due to a novel bioengineered polymerase, target sizes up to 12 kb can be amplified with a high fidelity.
Benefits of using VitaScript 1Step RT-PCR Kit
- proprietary 2-phase HotStart mechanism for maximum control over the reaction
- novel bioengineered phusion polymerase for highest figelity and long amplicons
- potent VitaScript Reverse Transcriptase for high sensitivity
- optimized buffer for GC-rich amplicons
Fig. Cover templates with GC-ranges from 40 to 80 % over different amplicon lengths.
Fig. VitaScript 1Step RT-PCR Kit detects as little as 1 fg input RNA.
SKU 25 rxn / 50 µl: PCCSKU1302
SKU 25 rxn / 50 µl: PCCSKU1303